A BODIPY-Based Donor/Donor-Acceptor System: Towards Highly Efficient Long-Wavelength-Excitable Near-IR Polymer Dots with Narrow and Strong Absorption Features.

Bright long-wavelength-excitable semiconducting polymer dots (LWE-Pdots) are highly desirable for in vivo imaging and multiplexed in vitro bioassays. LWE-Pdots have been obtained by incorporating a near-infrared (NIR) emitter into the backbone of a polymer host to develop a binary donor-acceptor (D-A) system. However, they usually suffer from severe concentration quenching and a trade-off between fluorescence quantum yield (Φ _f ) and absorption cross-section (σ). Herein, we describe a ternary component (D ₁ /D ₂ -A) strategy to achieve ultrabright, green laser-excitable Pdots with narrow-band NIR emission by introducing a BODIPY-based assistant polymer donor as D ₁ . The D ₁ /D ₂ -A Pdots possess improved Φ _f and σ compared to corresponding binary D ₂ -A Pdots. Their Φ _f is as high as 40.2 %, one of the most efficient NIR Pdots reported. The D ₁ /D ₂ -A Pdots show ultrahigh single-particle brightness, 83-fold brighter than Qdot 705 when excited by a 532 nm laser. When injected into mice, higher contrast in vivo tumor imaging was achieved using the ternary Pdots versus the binary D-A Pdots.
(© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)