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A clone coding for Schizophyllum commune beta-glucosidase: homology with a yeast beta-glucosidase.
- Source :
-
Biochemistry international [Biochem Int] 1986 Jun; Vol. 12 (6), pp. 905-12. - Publication Year :
- 1986
-
Abstract
- Three identical clones coding for a partial sequence of the Schizophyllum commune beta-glucosidase were isolated from a cDNA library in lambda gt11, using polyclonal antibody to the enzyme. The identity was confirmed by comparison of the amino-terminus of a peptide from a protease lys-C digest with the sequence inferred from the cDNA sequence. A comparison of the sequence with that reported for a beta-glucosidase from Candida pelliculosa revealed a region in the latter with 43% identity in amino acid sequence. There was also a similarity in the S. commune beta-glucosidase to an active site sequence proposed for a S. commune endoglucanase, suggesting the possibility of a common catalytic mechanism for these two glucolytic enzymes.
- Subjects :
- Amino Acid Sequence
Base Sequence
Genes
Genes, Fungal
Peptide Fragments analysis
Saccharomyces cerevisiae enzymology
Schizophyllum enzymology
Sequence Homology, Nucleic Acid
Species Specificity
Basidiomycota genetics
Cloning, Molecular
DNA metabolism
Glucosidases genetics
Saccharomyces cerevisiae genetics
Schizophyllum genetics
beta-Glucosidase genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0158-5231
- Volume :
- 12
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biochemistry international
- Publication Type :
- Academic Journal
- Accession number :
- 3091028