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Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato.
- Source :
-
PloS one [PLoS One] 2019 Mar 25; Vol. 14 (3), pp. e0214165. Date of Electronic Publication: 2019 Mar 25 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that Snakin-1 is involved in plant defense to pathogens as well as in plant growth and development, but its mechanism of action has not been completely elucidated yet. Here, we showed that leaves of Snakin-1 silenced potato transgenic plants exhibited increased levels of reactive oxygen species and significantly reduced content of ascorbic acid. Furthermore, Snakin-1 silencing enhanced salicylic acid content in accordance with an increased expression of SA-inducible PRs genes. Interestingly, gibberellic acid levels were also enhanced and transcriptome analysis revealed that a large number of genes related to sterol biosynthesis were downregulated in these silenced lines. Moreover, we demonstrated that Snakin-1 directly interacts with StDIM/DWF1, an enzyme involved in plant sterols biosynthesis. Additionally, the analysis of the expression pattern of PStSN1::GUS in potato showed that Snakin-1 is present mainly in young tissues associated with active growth and cell division zones. Our comprehensive analysis of Snakin-1 silenced lines demonstrated for the first time in potato that Snakin-1 plays a role in redox balance and participates in a complex crosstalk among different hormones.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Phytosterols biosynthesis
Phytosterols genetics
Plant Growth Regulators genetics
Plant Growth Regulators metabolism
Plant Leaves genetics
Plant Leaves metabolism
Plant Proteins biosynthesis
Plant Proteins genetics
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Solanum tuberosum genetics
Solanum tuberosum metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 14
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 30909287
- Full Text :
- https://doi.org/10.1371/journal.pone.0214165