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Chemiresistive DNA hybridization sensor with electrospun nanofibers: A method to minimize inter-device variability.
- Source :
-
Biosensors & bioelectronics [Biosens Bioelectron] 2019 May 15; Vol. 133, pp. 24-31. Date of Electronic Publication: 2019 Mar 18. - Publication Year :
- 2019
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Abstract
- Chemiresistive platforms are best suited for developing DNA hybridization detection systems, owing to their ease of fabrication, simple detection methodology and amenability towards electronics. In this work, we report development of a generic, robust, electrospun nanofiber based interdigitated chemiresistive platform for DNA hybridization detection. The platform comprises of interdigitated metal electrodes decorated with electrospun nanofibers on the top. Two approaches viz., drop casting of graphene doped Mn <subscript>2</subscript> O <subscript>3</subscript> nanofibers (GMnO) and direct electrospinning of polyaniline/polyethylene oxide (PANi/PEO) composite nanofibers, have been utilized to decorate these electrodes. In both approaches, inter-device variability, a key challenge for converting this proof-of-concept into a tangible prototype/product, has been addressed using a shadow masking technique. Consequently, the relative standard deviation for multiple PANi/PEO nanofiber based chemiresistors has been brought down from 17.82% (without shadow masking) to 4.41% (with shadow masking). The nanofibers are further modified with single-stranded probe DNAs, to capture a desired hybridization event. To establish the generic nature of the platform, detection of multiple target DNAs has been successfully demonstrated. These targets include dengue virus specific consensus primer (DENVCP) and four DNAs corresponding to Staphylococcus aureus specific genes, namely nuc, mecA, vanA and protein A. The chemiresistive detection of DENVCP has been performed in the concentration range of 10 fM - 1 µM, whereas the detection of the other targets has been carried out in the range of 1 pM - 1 µM. Using a 3σ method, we have estimated the limit of detection for the chemiresistive detection of DENVCP to be 1.9 fM.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Bacterial Proteins genetics
Bacterial Proteins isolation & purification
Carbon-Oxygen Ligases genetics
Carbon-Oxygen Ligases isolation & purification
DNA chemistry
DNA, Single-Stranded genetics
Graphite chemistry
Humans
Micrococcal Nuclease genetics
Micrococcal Nuclease isolation & purification
Nanofibers chemistry
Nucleic Acid Hybridization
Penicillin-Binding Proteins isolation & purification
Staphylococcal Protein A genetics
Staphylococcal Protein A isolation & purification
Staphylococcus aureus genetics
Biosensing Techniques
DNA isolation & purification
DNA, Single-Stranded chemistry
Staphylococcus aureus isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4235
- Volume :
- 133
- Database :
- MEDLINE
- Journal :
- Biosensors & bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 30903938
- Full Text :
- https://doi.org/10.1016/j.bios.2019.03.031