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Molecular identification and biological characteristic analysis of an Apiotrichum mycotoxinivorans (formerly Trichosporon mycotoxinivorans) strain isolated from sputum specimens of a pediatric patient with pneumonia.
- Source :
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Journal de mycologie medicale [J Mycol Med] 2019 Jun; Vol. 29 (2), pp. 120-126. Date of Electronic Publication: 2019 Mar 18. - Publication Year :
- 2019
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Abstract
- Apiotrichum mycotoxinivorans (formerly Trichosporon mycotoxinivorans) has long been used to degrade fungal toxins in livestock feed. However, clinic reports about this type of fungus are rare. In this study, we report the morphology, biochemistry, and molecular characteristics of an A. mycotoxinivorans strain isolated from a pediatric patient with congenital ventricular septal defect and pneumonia. A female patient, 26 months old, presented with congenital ventricular septal defect. Pulmonary infection symptoms were observed after the patient received cardiac repair surgery. Sputum bacterial and fungal cultures were positive for Elizabethkingia anophelis and a fungus, which was not readily identifiable using biochemical identification, or MALDI-TOF MS analysis. The strain was finally identified as A. mycotoxinivorans using amplification and sequencing of the D1/D2 region of 26S rDNA, ITS, and IGS1. Antifungal susceptibility test results suggested that fluconazole or voriconazole may be an appropriate choice for antifungal therapy. A biodegradability of ochratoxin A was considered as a characteristic of the fungal strain. Our results support the existing evidence that A. mycotoxinivorans is an opportunistic pathogen for human beings. Nucleic acid analysis allows for the accurate identification of the species in instances where conventional identification methods such as biochemical testing and MALDI-TOF MS may be unsuccessful.<br /> (Copyright © 2019. Published by Elsevier Masson SAS.)
- Subjects :
- Antifungal Agents pharmacology
Child, Preschool
DNA, Fungal genetics
Female
Humans
Mycological Typing Techniques
Phylogeny
Trichosporon drug effects
Trichosporonosis microbiology
Pneumonia microbiology
Sputum microbiology
Trichosporon classification
Trichosporon isolation & purification
Trichosporonosis diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1773-0449
- Volume :
- 29
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal de mycologie medicale
- Publication Type :
- Academic Journal
- Accession number :
- 30898449
- Full Text :
- https://doi.org/10.1016/j.mycmed.2019.01.010