Detection of carbapenemase-producing Pseudomonas aeruginosa: Evaluation of the carbapenem inactivation method (CIM).

Authors :: Gutiérrez S
Correa A
Hernández-Gómez C
De La Cadena E
Pallares C
Villegas MV
Source :: Enfermedades infecciosas y microbiologia clinica (English ed.) [Enferm Infecc Microbiol Clin (Engl Ed)] 2019 Dec; Vol. 37 (10), pp. 648-651. Date of Electronic Publication: 2019 Mar 18.
Publication Year :: 2019
Abstract: Introduction: The carbapenem inactivation method (CIM) is a cost-effective assay for detecting carbapenemases. However, its interpretation is unclear for Pseudomonas spp. We evaluate its accuracy when meropenem is changed to imipenem.<br />Methods: We analyzed 266 P. aeruginosa isolates. The CIM method consists of: resuspend bacterial colonies (a full 10μL loop) in 400μL water, in which a 10μg disk of meropenem/imipenem is immersed. After 2h of incubation (35°C), remove the disk, place it onto a Mueller-Hinton agar plate previously inoculated with Escherichia coli (ATCC 25922), and incubate at 35 ̊C between 18-24 h. Interpretation criteria (mm of inhibition zone): ≤19mm, positive; ≥25mm negative; 20-24mm, undetermined.<br />Results: Imipenem improves the sensitivity and specificity of CIM when compared to meropenem (99.4% and 98.9%, vs. 91.9% and 94.7%, respectively).<br />Conclusions: The accuracy of CIM for carbapenemase detection in P. aeruginosa is increased with the use of imipenem.<br /> (Copyright © 2019 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.)

Subjects :: Bacteriological Techniques methods
Humans
Microbial Sensitivity Tests methods
Anti-Bacterial Agents pharmacology
Bacterial Proteins biosynthesis
Carbapenems pharmacology
Meropenem pharmacology
Pseudomonas aeruginosa drug effects
Pseudomonas aeruginosa enzymology
beta-Lactamases biosynthesis

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