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Interferon-Beta Treatment Differentially Alters TLR2 and TLR4-Dependent Cytokine Production in Multiple Sclerosis Patients.
- Source :
-
Neuroimmunomodulation [Neuroimmunomodulation] 2019; Vol. 26 (2), pp. 77-83. Date of Electronic Publication: 2019 Mar 21. - Publication Year :
- 2019
-
Abstract
- Objective: Multiple sclerosis (MS) is a multifactorial chronic disease that affects the central nervous system (CNS). Toll-like receptors (TLRs) play a central role in cytokine production after pathogen- and danger-associated molecular patterns (PAMPs and DAMPs) and contribute to CNS damage in MS patients. Here, we evaluated the effects of interferon (IFN)-β treatment in TLR2 and TLR4-dependent cytokine production and mRNA expression in whole-blood cell cultures from MS patients.<br />Methods: We evaluated cytokine production by ELISA from whole-blood cell culture supernatants and mRNA expression by real-time polymerase chain reaction in peripheral blood mononuclear cells (PBMCs).<br />Results: In patients treated with IFN-β, tumor necrosis factor (TNF)-α production after exposure to TLR2 agonist (Pam3Cys) was lower than in healthy controls and untreated MS patients. However, IFN-β treatment had no significant effect on TNF-α production after TLR4 agonist (LPS) stimulation. On the other hand, interleukin (IL)-10 production was increased in TLR4- but not in TLR2-stimulated whole-blood cell culture from MS patients under IFN-β treatment when compared to the controls. No differences in TNF-α or IL-10 mRNA expression in PBMCs from healthy controls and untreated or treated MS patients were detected, although PBMCs from treated patients presented higher levels of IL-32γ mRNA than those from controls.<br />Conclusions: Our data suggest that IFN-β treatment alters the TLR-dependent immune response of PBMCs from MS patients. This may contribute to the beneficial effects of IFN-β treatment.<br /> (© 2019 S. Karger AG, Basel.)
- Subjects :
- Adult
Cytokines biosynthesis
Cytokines drug effects
Female
Humans
Immunologic Factors therapeutic use
Male
Middle Aged
Multiple Sclerosis drug therapy
Toll-Like Receptor 2 immunology
Toll-Like Receptor 4 immunology
Interferon-beta therapeutic use
Interleukin-10 biosynthesis
Multiple Sclerosis immunology
Tumor Necrosis Factor-alpha biosynthesis
Tumor Necrosis Factor-alpha drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0216
- Volume :
- 26
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Neuroimmunomodulation
- Publication Type :
- Academic Journal
- Accession number :
- 30897575
- Full Text :
- https://doi.org/10.1159/000495787