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Peimine suppresses interleukin‑1β‑induced inflammation via MAPK downregulation in chondrocytes.
- Source :
-
International journal of molecular medicine [Int J Mol Med] 2019 May; Vol. 43 (5), pp. 2241-2251. Date of Electronic Publication: 2019 Mar 21. - Publication Year :
- 2019
-
Abstract
- Osteoarthritis (OA) is the most common type of degenerative joint disease and secreted inflammatory molecules serve a pivotal role in it. Peimine has been reported to have anti‑inflammatory activity. In order to investigate the potential therapeutic role of Peimine in OA, mouse articular chondrocytes were treated with IL‑1β and different doses of Peimine in vitro. The data revealed that Peimine not only suppressed IL‑1β‑induced production of nitric oxide (NO) and prostaglandin E2, but also reduced the protein levels of inducible NO synthase (iNOS) and cyclooxygenase‑2 (COX‑2). In addition, Peimine inhibited the IL‑1β‑induced mRNA expression of matrix metalloproteinase (MMP)‑1, MMP‑3, MMP‑9, MMP‑13, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)‑4 and ADAMTS‑5. Furthermore, Peimine inhibited IL‑1β‑induced activation of the mitogen‑activated protein kinase (MAPK) pathway. The protective effect of Peimine on IL‑1β‑treated chondrocytes was attenuated following activation of the MAPK pathway, as demonstrated by the increased expression levels of MMP‑3, MMP‑13, ADAMTS‑5, iNOS and COX‑2 compared with the Peimine group. The in vivo data suggested that Peimine limited the development of OA in the mouse model. In general, the data indicate that Peimine suppresses IL‑1β‑induced inflammation in mouse chondrocytes by inhibiting the MAPK pathway, suggesting a promising therapeutic role for Peimine in the treatment of OA.
- Subjects :
- ADAMTS Proteins metabolism
Animals
Cartilage, Articular drug effects
Cartilage, Articular pathology
Cell Survival drug effects
Cevanes pharmacology
Chondrocytes drug effects
Cyclooxygenase 2 metabolism
Dinoprostone biosynthesis
Disease Models, Animal
Enzyme Activation drug effects
Inflammation pathology
Interleukin-1beta
MAP Kinase Signaling System drug effects
Matrix Metalloproteinases metabolism
Mice, Inbred C57BL
Nitric Oxide biosynthesis
Nitric Oxide Synthase Type II metabolism
Osteoarthritis pathology
Cevanes therapeutic use
Chondrocytes enzymology
Chondrocytes pathology
Down-Regulation
Inflammation enzymology
Mitogen-Activated Protein Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1791-244X
- Volume :
- 43
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- International journal of molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 30896805
- Full Text :
- https://doi.org/10.3892/ijmm.2019.4141