Back to Search
Start Over
Microbiological analysis of 5-formyltetrahydrofolic acid and other folates using an automatic 96-well plate reader.
- Source :
-
Analytical biochemistry [Anal Biochem] 1986 May 01; Vol. 154 (2), pp. 509-15. - Publication Year :
- 1986
-
Abstract
- The growth of auxotrophic bacteria remains the method of choice for the determination of biologically active folate metabolites in plasma. This report describes a microbiological assay for folates adapted to use disposable 96-well plates and an automatic plate reader. The modifications in the assay decreased reagent costs and made the analysis of hundreds of samples per day possible with a sensitivity limit of 10 fmol of (6S)-5-formyltetrahydrofolic acid. This limit compares favorably with that of previously reported, more laborious methods. The unnatural 6R diastereomer of 5-formyltetrahydrofolic acid did not interfere with the microbiological assay of the natural 6S diastereomer.
Details
- Language :
- English
- ISSN :
- 0003-2697
- Volume :
- 154
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Analytical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 3089059
- Full Text :
- https://doi.org/10.1016/0003-2697(86)90023-0