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Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice.

Authors :
Nagre N
Cong X
Pearson AC
Zhao X
Source :
Journal of visualized experiments : JoVE [J Vis Exp] 2019 Mar 02 (145). Date of Electronic Publication: 2019 Mar 02.
Publication Year :
2019

Abstract

Alveolar macrophages (AMs) guard the alveolar space of the lung. Phagocytosis by AMs plays a critical role in the defense against invading pathogens, the removal of dead cells or foreign particles, and in the resolution of inflammatory responses and tissue remodeling, processes that are mediated by various surface receptors of the AMs. Here, we report methods for the analysis of the phagocytic function of AMs using in vitro and in vivo assays and experimental strategies to differentiate between the pattern recognition receptor-, complement receptor-, and Fc gamma receptor-mediated phagocytosis. Finally, we discuss a method to establish and characterize a P. aeruginosa pneumonia model in mice to assess bacterial clearance in vivo. These assays represent the most common methods to evaluate AM functions and can also be used to study macrophage function and bacterial clearance in other organs.

Details

Language :
English
ISSN :
1940-087X
Issue :
145
Database :
MEDLINE
Journal :
Journal of visualized experiments : JoVE
Publication Type :
Academic Journal
Accession number :
30882784
Full Text :
https://doi.org/10.3791/59088