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Impaired GCR1 transcription resulted in defective inositol levels, vacuolar structure and autophagy in Saccharomyces cerevisiae.
- Source :
-
Current genetics [Curr Genet] 2019 Aug; Vol. 65 (4), pp. 995-1014. Date of Electronic Publication: 2019 Mar 16. - Publication Year :
- 2019
-
Abstract
- In yeast, the GCR1 transcription factor is involved in the regulation of glycolysis and its deletion exhibited growth defect, reduced inositol and phosphatidylinositol (PI) levels compared to WT cells. We observed a down regulation of the INO1 and PIS1 expression in gcr1∆ cells under both I- and I+ conditions and the over expression of GCR1 in gcr1∆ cells restored the growth, retrieved the expression of INO1, and PIS1 comparable to WT cells. In the gel shift assay, the Gcr1p binds to its consensus sequence CTTCC in PIS1 promoter and regulates its expression but not in INO1 transcription. The WT cells, under I- significantly reduced the expression of GCR1 and PIS1, but increased the expression of KCS1 and de-repressed INO1. The Kcs1p expression was reduced in gcr1∆ cells; this reduced INO1 expression resulting in abnormal vacuolar structure and reduced autophagy in Saccharomyces cerevisiae.
- Subjects :
- Binding Sites
DNA-Binding Proteins chemistry
Gene Expression Regulation, Fungal genetics
Glycolysis genetics
Inositol genetics
Inositol metabolism
Promoter Regions, Genetic
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins chemistry
Transcription Factors chemistry
Vacuoles genetics
Vacuoles ultrastructure
Autophagy genetics
DNA-Binding Proteins genetics
Saccharomyces cerevisiae Proteins genetics
Transcription Factors genetics
Transcription, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0983
- Volume :
- 65
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Current genetics
- Publication Type :
- Academic Journal
- Accession number :
- 30879088
- Full Text :
- https://doi.org/10.1007/s00294-019-00954-2