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Regulation of Microcystin-LR-Induced DNA Damage by miR-451a in HL7702 Cells.
- Source :
-
Toxins [Toxins (Basel)] 2019 Mar 15; Vol. 11 (3). Date of Electronic Publication: 2019 Mar 15. - Publication Year :
- 2019
-
Abstract
- Microcystin-LR is a cyclic heptapeptide hepatotoxin produced by harmful cyanobacteria. A panel of microRNAs containing miR-451a were found to be significantly changed in normal human liver cells HL7702 after exposure to microcystin-LR (MC-LR) in our previous study. However, the functions of miR-451a in hepatotoxicity induced by MC-LR remained unclear. The study aimed to investigate the impacts of miR-451a in HL7702 cells following treatment with 5 or 10 μM MC-LR. The comet assay indicated that MC-LR can influence Olive tail moment (OTM) in HL7702 cells. Furthermore, increase of miR-451a significantly repressed DNA damage and the protein expression level of γ-H2AX induced by MC-LR. Moreover, over-expression of miR-451a inhibited the expression level of p-AKT1 protein in cells following treatment by MC-LR. These results showed that miR-451a may protect from MC-LR-induced DNA damage by down-regulating the expression of p-AKT1, which provides new clues for the diagnosis and therapy policies for liver damage induced by MC-LR.
Details
- Language :
- English
- ISSN :
- 2072-6651
- Volume :
- 11
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Toxins
- Publication Type :
- Academic Journal
- Accession number :
- 30875960
- Full Text :
- https://doi.org/10.3390/toxins11030164