Bioactive 2-(Methyldithio)Pyridine-3-Carbonitrile from Persian Shallot ( Allium stipitatum Regel.) Exerts Broad-Spectrum Antimicrobial Activity.

Authors :: Karunanidhi A
Ghaznavi-Rad E
Jeevajothi Nathan J
Joseph N
Chigurupati S
Mohd Fauzi F
Pichika MR
Hamat RA
Lung LTT
van Belkum A
Neela V
Source :: Molecules (Basel, Switzerland) [Molecules] 2019 Mar 13; Vol. 24 (6). Date of Electronic Publication: 2019 Mar 13.
Publication Year :: 2019
Abstract: Antibiotic resistance is a problem that continues to challenge the healthcare sector, especially in clinically significant pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Herein is described the isolation and structure elucidation of a bioactive compound from Allium stipitatum with antimicrobial activity. Crude Allium stipitatum dichloromethane extract (ASDE) was subjected to systematic purification by chromatographic procedures to afford various bioactive fractions. A fraction that exhibited anti-MRSA activity (4 µg·mL <superscript>-1</superscript> ) was further characterized to determine the structure. The structure of the compound was elucidated as 2-(methyldithio)pyridine-3-carbonitrile (2-Medpy-3-CN). The 2-Medpy-3-CN compound, which was screened for antimicrobial activity, exhibited minimum inhibitory concentrations (MICs) in the range of 0.5 to >64 µg·mL <superscript>-1</superscript> for tested bacterial species and 0.25 to 2 µg·mL <superscript>-1</superscript> for Candida spp. Further studies are important to confirm the drug target and mechanism of action.

Subjects :: Anti-Bacterial Agents pharmacology
Candida drug effects
Chemical Fractionation
Microbial Sensitivity Tests
Molecular Structure
Nitriles chemistry
Nitriles isolation & purification
Phytochemicals analysis
Pyridines isolation & purification
Pyridines pharmacology
Anti-Bacterial Agents chemistry
Methicillin-Resistant Staphylococcus aureus drug effects
Pyridines chemistry
Shallots chemistry

Full Text Access

Tools