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A Hox-TALE regulatory circuit for neural crest patterning is conserved across vertebrates.
- Source :
-
Nature communications [Nat Commun] 2019 Mar 13; Vol. 10 (1), pp. 1189. Date of Electronic Publication: 2019 Mar 13. - Publication Year :
- 2019
-
Abstract
- In jawed vertebrates (gnathostomes), Hox genes play an important role in patterning head and jaw formation, but mechanisms coupling Hox genes to neural crest (NC) are unknown. Here we use cross-species regulatory comparisons between gnathostomes and lamprey, a jawless extant vertebrate, to investigate conserved ancestral mechanisms regulating Hox2 genes in NC. Gnathostome Hoxa2 and Hoxb2 NC enhancers mediate equivalent NC expression in lamprey and gnathostomes, revealing ancient conservation of Hox upstream regulatory components in NC. In characterizing a lamprey hoxα2 NC/hindbrain enhancer, we identify essential Meis, Pbx, and Hox binding sites that are functionally conserved within Hoxa2/Hoxb2 NC enhancers. This suggests that the lamprey hoxα2 enhancer retains ancestral activity and that Hoxa2/Hoxb2 NC enhancers are ancient paralogues, which diverged in hindbrain and NC activities. This identifies an ancestral mechanism for Hox2 NC regulation involving a Hox-TALE regulatory circuit, potentiated by inputs from Meis and Pbx proteins and Hox auto-/cross-regulatory interactions.
- Subjects :
- Animals
Animals, Genetically Modified
Binding Sites genetics
Cell Line
Conserved Sequence physiology
Enhancer Elements, Genetic genetics
Homeodomain Proteins genetics
Lampreys
Mice
Mouse Embryonic Stem Cells
Neural Crest metabolism
Sequence Alignment
Vertebrates embryology
Zebrafish
Gene Expression Regulation, Developmental physiology
Genes, Homeobox physiology
Homeodomain Proteins metabolism
Neural Crest embryology
Vertebrates genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 30867425
- Full Text :
- https://doi.org/10.1038/s41467-019-09197-8