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Bone marrow-derived mesenchymal stem cells attenuate severe acute pancreatitis via regulation of microRNA-9 to inhibit necroptosis in rats.
- Source :
-
Life sciences [Life Sci] 2019 Apr 15; Vol. 223, pp. 9-21. Date of Electronic Publication: 2019 Mar 09. - Publication Year :
- 2019
-
Abstract
- Aims: Severe acute pancreatitis (SAP) is an acute disease of the digestive system accompanied by pancreatic necrosis. We have found that bone marrow-derived mesenchymal stem cells (BMSCs) can attenuate SAP, but the underlying mechanism remains unclear. The present study was conducted to explore the possible mechanisms by which BMSCs alleviate SAP.<br />Main Methods: BMSCs and BMSCs engineered to overexpress microRNA (miR)-9 (miR-9-BMSCs) were transplanted into rat models of SAP via the tail vein. Pancreatic acinar cells (PACs) were isolated from rat pancreatic tissues and induced by tumor necrosis factor-α (TNF-α) in vitro.<br />Key Findings: miR-9-BMSCs significantly reduced the systemic inflammatory response, impeded the necroptosis signaling pathway and promoted regeneration of damaged pancreas in vivo. miR-9-BMSCs secreted miR-9, which targeted the gene encoding receptor interacting protein kinase 1 in PACs induced by TNF-α, to inhibit necroptosis and ameliorate SAP.<br />Significance: miR-9-BMSCs can reduce SAP-induced injury to pancreatic tissues and PACs by regulating miR-9 to suppress necroptosis.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Amylases blood
Animals
Cytokines blood
Humans
Lipase blood
Male
Necrosis
Pancreatitis, Acute Necrotizing genetics
Pancreatitis, Acute Necrotizing pathology
Rats
Rats, Sprague-Dawley
Apoptosis genetics
Bone Marrow Cells cytology
Gene Expression Regulation
Mesenchymal Stem Cell Transplantation
MicroRNAs genetics
Pancreatitis, Acute Necrotizing therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0631
- Volume :
- 223
- Database :
- MEDLINE
- Journal :
- Life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 30862569
- Full Text :
- https://doi.org/10.1016/j.lfs.2019.03.019