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Addressing the constraints of Tritrichomonas foetus sample collection in remote areas: lyophilized modified Diamond's media as a substitute for liquid medium.
- Source :
-
Parasitology [Parasitology] 2019 Aug; Vol. 146 (9), pp. 1184-1187. Date of Electronic Publication: 2019 Mar 12. - Publication Year :
- 2019
-
Abstract
- Bovine trichomoniasis is a notifiable, reproductive disease of cattle caused by the parasite Tritrichomonas foetus. Culturing with modified Diamond's medium (MDM) is required to increase the low number of organisms received from a preputial sample, but is limited in application to remote areas as it requires continuous cold chain storage. This study utilized lyophilization to sustain the viability of MDM during transport in lieu of a continuous cold chain. All lyophilized MDM was able to sustain T. foetus after storage for 42 days at 24 °C, and the results demonstrated that lyophilized MDM was equally as viable as refrigerated liquid MDM. Storage of lyophilized MDM at room temperature for 1 and 7 days did not impact T. foetus yield, both with and without exposure to light. A limitation of the lyophilized MDM was demonstrated with a significant decrease in T. foetus yield when the media was stored at 37 and 58 °C. The lyophilization of MDM provides a robust method of transporting and storing medium prior to reconstitution and inoculation, for use in T. foetus diagnosis and surveillance in remote areas.
- Subjects :
- Animals
Australia
Cattle
Cattle Diseases parasitology
Freeze Drying
Temperature
Trichomonas Infections diagnosis
Tritrichomonas foetus isolation & purification
Cattle Diseases diagnosis
Culture Media chemistry
Protozoan Infections, Animal diagnosis
Specimen Handling methods
Trichomonas Infections veterinary
Tritrichomonas foetus growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8161
- Volume :
- 146
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 30859924
- Full Text :
- https://doi.org/10.1017/S0031182019000258