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Hydrogen ventilation combined with mild hypothermia improves short-term neurological outcomes in a 5-day neonatal hypoxia-ischaemia piglet model.
- Source :
-
Scientific reports [Sci Rep] 2019 Mar 11; Vol. 9 (1), pp. 4088. Date of Electronic Publication: 2019 Mar 11. - Publication Year :
- 2019
-
Abstract
- Despite its poor outcomes, therapeutic hypothermia (TH) is the current standard treatment for neonatal hypoxic-ischaemic encephalopathy (HIE). In this study, due to its antioxidant, anti-inflammatory, and antiapoptotic properties, the effectiveness of molecular hydrogen (H <subscript>2</subscript> ) combined with TH was evaluated by means of neurological and histological assessments. Piglets were divided into three groups: hypoxic-ischaemic insult with normothermia (NT), insult with hypothermia (TH, 33.5 ± 0.5 °C), and insult with hypothermia with H <subscript>2</subscript> ventilation (TH-H <subscript>2</subscript> , 2.1-2.7%). H <subscript>2</subscript> ventilation and TH were administered for 24 h. After ventilator weaning, neurological assessment was performed every 6 h for 5 days. On day 5, the brains of the piglets were harvested for histopathological analysis. Regarding the neurological score, the piglets in the TH-H <subscript>2</subscript> group consistently had the highest score from day 2 to 5 and showed a significantly higher neurological score from day 3 compared with the NT group. Most piglets in the TH-H <subscript>2</subscript> group could walk at day 3 of recovery, whereas walking ability was delayed in the two other groups. The histological results revealed that TH-H <subscript>2</subscript> tended to improve the status of cortical gray matter and subcortical white matter, with a considerable reduction in cell death. In this study, the combination of TH and H <subscript>2</subscript> improved short-term neurological outcomes in neonatal hypoxic-ischaemic piglets.
- Subjects :
- Animals
Animals, Newborn
Antioxidants pharmacology
Asphyxia Neonatorum drug therapy
Asphyxia Neonatorum physiopathology
Brain physiology
Disease Models, Animal
Humans
Hypoxia-Ischemia, Brain physiopathology
Infant, Newborn
Respiration
Swine
Ventilation methods
White Matter drug effects
White Matter physiopathology
Brain drug effects
Hydrogen pharmacology
Hypothermia, Induced methods
Hypoxia-Ischemia, Brain drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 30858437
- Full Text :
- https://doi.org/10.1038/s41598-019-40674-8