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Genotypic and phenotypic characterization of Edwardsiella isolates from different fish species and geographical areas in Asia: Implications for vaccine development.
- Source :
-
Journal of fish diseases [J Fish Dis] 2019 Jun; Vol. 42 (6), pp. 835-850. Date of Electronic Publication: 2019 Mar 08. - Publication Year :
- 2019
-
Abstract
- The genus Edwardsiella is one of the major causes of fish diseases globally. Herein, we examined 37 isolates from ten different fish species from India, South Korea and Taiwan to gain insight into their phenotypic and genotypic properties, of which 30 were characterized as E. tarda with phenotypic homology estimated at 85.71% based on API-20E biochemical tests. Genotyping using 16S rRNA put all isolates together with E. anguillarum, E. hoshinae, E. tarda, E. piscicida and E. ictaluri reference strains in a monophyletic group. In contrast, the gyrB phylogenetic tree clearly separated E. ictaluri, E. tarda and E. hoshinae reference strains from our isolates and put our isolates into two groups with group I being homologous with the E. anguillarum reference strain while group II was homologous with the E. piscicida reference strain. Hence, our findings point to E. piscicida and E. anguillarum as species infecting different fish species in Asia. Homology of the ompW protein suggested that strains with broad protective coverage could be identified as vaccine candidates. This study underscores the importance of combining genotyping with phenotyping for valid species classification. In addition, it accentuates the importance of phylogenetic comparison of bacterial antigens for identification of potential vaccine candidates.<br /> (© 2019 John Wiley & Sons Ltd.)
- Subjects :
- Animals
Aquaculture
Asia epidemiology
Bacterial Vaccines
DNA, Bacterial genetics
Disease Outbreaks
Edwardsiella isolation & purification
Edwardsiella tarda genetics
Enterobacteriaceae Infections epidemiology
Fish Diseases epidemiology
Genotype
Geography
India epidemiology
Phenotype
Phylogeny
RNA, Ribosomal, 16S genetics
Seafood microbiology
Sequence Analysis, DNA
Edwardsiella genetics
Enterobacteriaceae Infections veterinary
Fish Diseases microbiology
Fishes microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2761
- Volume :
- 42
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of fish diseases
- Publication Type :
- Academic Journal
- Accession number :
- 30851008
- Full Text :
- https://doi.org/10.1111/jfd.12984