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Autoantibodies to intermediate filaments in acute viral hepatitis A, B and non-A, non-B are directed against vimentin.
- Source :
-
Journal of clinical & laboratory immunology [J Clin Lab Immunol] 1986 Jan; Vol. 19 (1), pp. 1-4. - Publication Year :
- 1986
-
Abstract
- Using ELISA and immunoblotting, autoantibodies to vimentin were sought in sera from 10 patients with acute hepatitis A, 10 with acute hepatitis B, 13 with acute non-A, non-B hepatitis, 16 with autoimmune chronic active hepatitis, 17 with cytomegalovirus infection and 40 from healthy persons. The ELISA results were expressed as a percentage of the value obtained with a monoclonal antibody to vimentin. The results (mean and SD) for acute hepatitis A (51.2 +/- 21.7%), acute hepatitis B (44.5 +/- 28.2%) and acute hepatitis non-A, non-B (43.0 +/- 16.4%) were significantly (p less than 0.01) higher than those for autoimmune chronic active hepatitis (20.5 +/- 7.7%), cytomegalovirus infection (25.9 +/- 12.2%) and healthy controls (16.8 +/- 9.3%). Immunoblotting showed that sera from patients with acute viral hepatitis reacted with 57 kd vimentin in triton-cytoskeletal extracts of fibroblasts. These results show that autoantibodies to vimentin are present in sera from patients with acute hepatitis A, B and non-A, non-B. Antivimentin autoantibodies may be useful in the diagnosis of acute non-A, non-B hepatitis.
- Subjects :
- Autoantibodies immunology
Collodion
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Humans
Paper
Autoantibodies analysis
Cytoskeleton immunology
Hepatitis A immunology
Hepatitis B immunology
Hepatitis C immunology
Hepatitis, Viral, Human immunology
Intermediate Filaments immunology
Vimentin immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0141-2760
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of clinical & laboratory immunology
- Publication Type :
- Academic Journal
- Accession number :
- 3084793