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IP-10 dried blood spots assay monitoring treatment efficacy in extrapulmonary tuberculosis in a low-resource setting.
- Source :
-
Scientific reports [Sci Rep] 2019 Mar 07; Vol. 9 (1), pp. 3871. Date of Electronic Publication: 2019 Mar 07. - Publication Year :
- 2019
-
Abstract
- Treatment efficacy is difficult to evaluate in extrapulmonary tuberculosis (EPTB) patients. Interferon-γ inducible protein (IP-)10 has been suggested as a biomarker for response to treatment. We have investigated if IP-10 from dried plasma spots (DPS) or dried blood spots (DBS) can be used in treatment monitoring of EPTB patients in a low-resource setting of Zanzibar. IP-10 levels in plasma, DPS and DBS samples collected before, during (2 months) and after TB treatment of 36 EPTB patients (6 culture and/or Xpert MTB/RIF positive and 30 clinically diagnosed) and 8 pulmonary tuberculosis (PTB) patients, were quantified by an enzyme-linked immunosorbent assay. There was a high positive correlation between IP-10 measured in plasma and DPS and DBS, respectively. We found a significant decline in IP-10 levels from baseline to end of treatment in plasma, DPS and DBS, both in EPTB and PTB patients. The declines were observed already after 2 months in HIV negative patients. In conclusion, the DPS/DBS IP-10 assay allows for easy and manageable monitoring in low-resource settings and our findings suggest that IP-10 may serve as a biomarker for treatment efficacy in EPTB patients, albeit further studies in cohorts of patients with treatment failure and relapse are needed.
- Subjects :
- Adolescent
Adult
Aged
Antitubercular Agents therapeutic use
Biomarkers blood
Coinfection blood
Enzyme-Linked Immunosorbent Assay
Female
HIV Infections blood
HIV Infections complications
Humans
Longitudinal Studies
Male
Middle Aged
Plasma chemistry
Treatment Outcome
Tuberculosis complications
Tuberculosis drug therapy
Tuberculosis economics
Young Adult
Chemokine CXCL10 blood
Dried Blood Spot Testing economics
Dried Blood Spot Testing methods
Tuberculosis blood
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 30846768
- Full Text :
- https://doi.org/10.1038/s41598-019-40458-0