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MicroRNA-575 regulates development of gastric cancer by targeting PTEN.
- Source :
-
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2019 May; Vol. 113, pp. 108716. Date of Electronic Publication: 2019 Mar 04. - Publication Year :
- 2019
-
Abstract
- MicroRNA-575 (miR-575) is oncogene in many tumors. However, the role of miR-575 in the progression of gastric cancer (GC) is still unknown. The aim of this study was to identify whether miR-575 play a role in the development of GC. We obtained GC cell lines, GC tissues from 40 patients to measure the levels of miR-575 and its predicted target PTEN by using RT‑PCR, immunohistochemistry or western blot analysis. MGC-803 cells were transfected with miR-575 inhibitor, and cells viability and apoptosis were measured. miR-575 aberrantly up-regulated in GC tissues and GC cell lines compared with corresponding control. In cell lines, MGC‑803 expressed highest level of miR-575 among the tested cells. The level of miR-575 was correlated with the tumor size, AJCC stage and prognosis, but not with the other clinical parameters. Knockdown of miR-575 inhibited proliferation and promoted apoptotic death of MGC-803 cells both in vitro and vivo. PTEN levels (both mRNA and protein) were remarkably decreased in cancer tissues compared with the paired-adjacent tissues, and negatively correlated with miR-575 in the tissues. By using luciferase reporter assay, we found PTEN was a direct downstream target of miR-575, and negatively regulated by miR-575 via targeting its 3'UTR. Knockdown or overexpression of miR-575 in MGC-803 cells negatively regulated PTEN expression. Finally, silencing PTEN partially impaired anti-proliferative effects of miR-575 inhibitor. miRNA-575 serves a pivotal role in GC as a cancer promoter gene by targeting PTEN to regulate proliferation and apoptosis of the cancer cells.<br /> (Copyright © 2019 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Subjects :
- Animals
Cell Line, Tumor
Cell Survival genetics
Female
Gene Expression Regulation, Neoplastic
Humans
Male
Mice, Inbred BALB C
Mice, Nude
Middle Aged
Neoplasm Invasiveness
PTEN Phosphohydrolase genetics
Stomach Neoplasms genetics
Stomach Neoplasms metabolism
Xenograft Model Antitumor Assays
Apoptosis genetics
Cell Proliferation genetics
MicroRNAs genetics
PTEN Phosphohydrolase metabolism
Stomach Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6007
- Volume :
- 113
- Database :
- MEDLINE
- Journal :
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Publication Type :
- Academic Journal
- Accession number :
- 30844657
- Full Text :
- https://doi.org/10.1016/j.biopha.2019.108716