Back to Search
Start Over
Porcine lactoferrin-derived peptide LFP-20 modulates immune homoeostasis to defend lipopolysaccharide-triggered intestinal inflammation in mice.
- Source :
-
The British journal of nutrition [Br J Nutr] 2019 Jun; Vol. 121 (11), pp. 1255-1263. Date of Electronic Publication: 2019 Mar 06. - Publication Year :
- 2019
-
Abstract
- The performance of immune system is vital for defending the body from pathogens, and it plays a crucial role in health homoeostasis. In a previous study, we have shown that LFP-20, a twenty-amino acid antimicrobial peptide in the N terminus of porcine lactoferrin, modulated inflammatory response in colitis. Here, we further investigated the effects of LFP-20 on immune homoeostasis to elucidate the mechanism of its anti-inflammation action. A lipopolysaccharide (LPS)-triggered systemic inflammatory response mice model was established. On the basis of observed mucosal lesions and apoptosis in small intestine, we found increased macrophage and neutrophil infiltration in ileum after LPS stimulation. Expectedly, LFP-20 pre-treatment attenuated the LPS-mediated immune disorders in ileum. Moreover, the flow cytometry results indicated pre-treatment with LFP-20 sustained the balance of CD3+CD8+ T cells, B cells and natural killer cells in LPS-triggered immune disturbance. Simultaneously, we demonstrated LFP-20 modulated the secretion of both activated Th1-related IL-12p70, interferon-γ, TNF-α and Th2-related IL-4, IL-5 and IL-6. Furthermore, we found LFP-20 facilitated a balanced Th1 and Th2 response, which triggered cellular defence mechanisms and induced B cells to produce opsonising antibodies belonging to certain IgG subclasses to defend against LPS stimulation. Collectively, our study indicated pre-treatment with LFP-20 could defend against LPS-triggered systemic inflammatory response in mice via modulating immune homoeostasis.
- Subjects :
- Animals
Cytokines metabolism
Disease Models, Animal
Ileitis chemically induced
Ileum immunology
Lipopolysaccharides
Lymphocyte Activation drug effects
Macrophages immunology
Mice
Neutrophils immunology
Anti-Inflammatory Agents pharmacology
Homeostasis drug effects
Ileitis immunology
Immunity, Active drug effects
Lactoferrin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1475-2662
- Volume :
- 121
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The British journal of nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 30837028
- Full Text :
- https://doi.org/10.1017/S0007114519000485