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Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2019 Mar 04; Vol. 20 (5). Date of Electronic Publication: 2019 Mar 04. - Publication Year :
- 2019
-
Abstract
- A deeper understanding of the detailed mechanism of in vivo tissue healing is necessary for the development of novel regenerative therapies. Among several external factors, environmental pH is one of the crucial parameters that greatly affects enzyme activity and cellular biochemical reactions involving tissue repair and homeostasis. In this study, in order to analyze the microenvironmental conditions during bone healing, we first measured the pH in vivo at the bone healing site using a high-resolution fiber optic pH microsensor directly in femur defects and tooth extraction sockets. The pH was shown to decrease from physiological 7.4 to 6.8 during the initial two days of healing (inflammatory phase). In the same initial stages of the inflammatory phase of the bone healing process, mesenchymal stem cells (MSCs) are known to migrate to the healing site to contribute to tissue repair. Therefore, we investigated the effect of a short-term acidic (pH 6.8) pre-treatment on the stemness of bone marrow-derived MSCs (BMSCs). Interestingly, the results showed that pre-treatment of BMSCs with acidic pH enhances the expression of stem cell markers (OCT-4, NANOG, SSEA-4), as well as cell viability and proliferation. On the other hand, acidic pH decreased BMSC migration ability. These results indicate that acidic pH during the initial stages of bone healing is important to enhance the stem cell properties of BMSCs. These findings may enable the development of novel methods for optimization of stem cell function towards tissue engineering or regenerative medicine.
- Subjects :
- Animals
Bone Marrow Cells cytology
Bone Marrow Cells drug effects
Bone Regeneration drug effects
Cell Differentiation drug effects
Cell Proliferation drug effects
Cell Survival drug effects
Cellular Microenvironment drug effects
Humans
Hydrogen-Ion Concentration drug effects
Mesenchymal Stem Cell Transplantation
Mesenchymal Stem Cells cytology
Mesenchymal Stem Cells drug effects
Mice
Nanog Homeobox Protein genetics
Octamer Transcription Factor-3 genetics
Regenerative Medicine
Stage-Specific Embryonic Antigens genetics
Stem Cells cytology
Stem Cells drug effects
Wound Healing genetics
Acids pharmacology
Bone Regeneration genetics
Osteogenesis drug effects
Tissue Engineering methods
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 20
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 30836626
- Full Text :
- https://doi.org/10.3390/ijms20051097