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Redox-responsive chitosan oligosaccharide-SS-Octadecylamine polymeric carrier for efficient anti-Hepatitis B Virus gene therapy.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2019 May 15; Vol. 212, pp. 215-221. Date of Electronic Publication: 2019 Feb 15. - Publication Year :
- 2019
-
Abstract
- DrzBC and DrzBS (10-23 DNAzyme) could block the expression of HBV e- and s- gene respectively. But the application of 10-23 DNAzyme was limited owing to the lack of appropriate delivery vehicles. Chitosan oligosaccharide-SS-Octadecylamine (CSSO), a redox-responsive nano-sized polymeric carrier, could self-aggregate and bind with DNA by electrostatic interaction at proper mass ratio. Compared with the traditional commercial carrier Lipo2000, CSSO exhibited lower cytotoxicity, efficient cellular uptake by targeting cells, and rapidly DNA released in cytoplasm after escaping from endosomes. Including the same DNA concentration, Lipo2000/(DrzBC or DrzBS) showed maximum inhibitory rate on HBeAg (47.29 ± 1.86%) and HBsAg (33.58 ± 0.72%) secretion after 48 h incubation, and then both decreased. In contrast, HBeAg secretion inhibition by CSSO/DrzBC and HBsAg secretion inhibition by CSSO/DrzBS were up to 73.86 ± 1.77% and 67.80 ± 2.51% at 48 h, and further increased to 83.83 ± 2.34% and 76.79 ± 2.18% at 72 h, respectively. CSSO is a promising redox-responsive polymeric carrier for efficient anti-Hepatitis B Virus gene therapy.<br /> (Copyright © 2019. Published by Elsevier Ltd.)
- Subjects :
- Amines metabolism
Chitosan metabolism
DNA, Viral drug effects
DNA, Viral genetics
DNA, Viral metabolism
Dose-Response Relationship, Drug
Drug Carriers administration & dosage
Drug Carriers metabolism
Hep G2 Cells
Hepatitis B virus genetics
Hepatitis B virus metabolism
Humans
Oligosaccharides metabolism
Oxidation-Reduction drug effects
Polymers metabolism
Amines administration & dosage
Chitosan administration & dosage
Genetic Therapy methods
Hepatitis B virus drug effects
Oligosaccharides administration & dosage
Polymers administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 212
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 30832850
- Full Text :
- https://doi.org/10.1016/j.carbpol.2019.02.047