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Effects of methyl mercuric chloride and sulfhydryl inhibitors on phospholipid synthetic activity of lymphocytes.
- Source :
-
Journal of applied toxicology : JAT [J Appl Toxicol] 1986 Feb; Vol. 6 (1), pp. 49-53. - Publication Year :
- 1986
-
Abstract
- The effect of methyl mercuric chloride on the activity of phospholipid synthesis of rat lymph node lymphocytes was compared with that of sulfhydryl inhibitors. Measurement of the radioactivity of [14C]oleic acid and [14C]acetate incorporated into lecithin of cells during short-term incubation showed that all the inhibitors tested similarly reduced the incorporation. However, methyl mercuric chloride (MMC) was the strongest inhibitor, being effective at 4 microM and causing more than 80% decrease at 20 microM. Inhibition by the sulfhydryl inhibitors, at less than 40 microM, ranked as follows: N-ethylmaleimide greater than alpha-chloroacetophenone greater than hydroquinone greater than iodoacetamide. MMC also obstructed the enhancement by phytohemagglutinin of [14C]oleic acid incorporation into lecithin. MMC was effective at 2 microM, while the other agents had little or no effect at this concentration. Further investigation suggested that inhibition of phospholipid synthesis did not depend on reduced incorporation of oleic acid into the cellular membrane but on decreased turnover of the fatty acid into phospholipids after the incorporation. The viability of lymphocytes incubated with the agents was measured by trypan blue dye-exclusion test. More than 90% of the cells treated with MMC at a concentration as low as 20-40 microM died, but the SH inhibitors, including NEM which greatly inhibited the phospholipid synthesis, produced few cell deaths at these concentrations. These observations show that the SH inhibitors affect enzymes in phospholipid synthesis, whereas MMC not only inhibits the enzymes but kills cells.
- Subjects :
- Animals
Cells, Cultured
Dose-Response Relationship, Drug
Ethylmaleimide metabolism
Ethylmaleimide pharmacology
Hydroquinones metabolism
Hydroquinones pharmacology
Iodoacetamide metabolism
Iodoacetamide pharmacology
Lymphocyte Activation drug effects
Lymphocytes drug effects
Male
Oleic Acid
Oleic Acids metabolism
Oleic Acids pharmacology
Rats
Rats, Inbred Strains
omega-Chloroacetophenone metabolism
omega-Chloroacetophenone pharmacology
Lymphocytes metabolism
Methylmercury Compounds pharmacology
Phospholipids biosynthesis
Sulfhydryl Reagents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0260-437X
- Volume :
- 6
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of applied toxicology : JAT
- Publication Type :
- Academic Journal
- Accession number :
- 3082964
- Full Text :
- https://doi.org/10.1002/jat.2550060111