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Transcription-dependent spreading of the Dal80 yeast GATA factor across the body of highly expressed genes.
- Source :
-
PLoS genetics [PLoS Genet] 2019 Feb 28; Vol. 15 (2), pp. e1007999. Date of Electronic Publication: 2019 Feb 28 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- GATA transcription factors are highly conserved among eukaryotes and play roles in transcription of genes implicated in cancer progression and hematopoiesis. However, although their consensus binding sites have been well defined in vitro, the in vivo selectivity for recognition by GATA factors remains poorly characterized. Using ChIP-Seq, we identified the Dal80 GATA factor targets in yeast. Our data reveal Dal80 binding to a large set of promoters, sometimes independently of GATA sites, correlating with nitrogen- and/or Dal80-sensitive gene expression. Strikingly, Dal80 was also detected across the body of promoter-bound genes, correlating with high expression. Mechanistic single-gene experiments showed that Dal80 spreading across gene bodies requires active transcription. Consistently, Dal80 co-immunoprecipitated with the initiating and post-initiation forms of RNA Polymerase II. Our work suggests that GATA factors could play dual, synergistic roles during transcription initiation and post-initiation steps, promoting efficient remodeling of the gene expression program in response to environmental changes.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Binding Sites
Chromatin Immunoprecipitation
DNA, Fungal chemistry
Gene Expression Regulation, Fungal
RNA Polymerase II metabolism
Sequence Analysis, RNA
Transcription, Genetic
DNA, Fungal metabolism
GATA Transcription Factors metabolism
Promoter Regions, Genetic
Repressor Proteins metabolism
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins metabolism
Up-Regulation
Subjects
Details
- Language :
- English
- ISSN :
- 1553-7404
- Volume :
- 15
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- PLoS genetics
- Publication Type :
- Academic Journal
- Accession number :
- 30818362
- Full Text :
- https://doi.org/10.1371/journal.pgen.1007999