Novelty in limbal stem cell culture and cell senescence.

Authors :: Boroumand N
Nosrati Tirkani A
Javid D
Hasani A
Taherzadeh D
Hosseinzadeh A
Nooripour S
Zarei-Ghanavati S
Hashemy SI
Alamdari DH
Source :: Experimental eye research [Exp Eye Res] 2019 Apr; Vol. 181, pp. 294-301. Date of Electronic Publication: 2019 Feb 23.
Publication Year :: 2019
Abstract: Limbal stem cell deficiency is a pathological state. Recently, limbal stem cell (LSC) transplantation has attracted great interest as a therapeutic method which mainly involves in-vitro expansion of LSCs. It is believed that ex-vivo cultivation conditions could affect the outcome of surgery and the rate of successful transplantation. Thus, we aimed to define a suitable culture condition by conducting a research on ex-vivo expanded LSCs to maintain an optimized graft of amniotic membrane with cultivated-limbal stem cells, regarding the quantity and quality, with the hope of improving the clinical outcome.<br /> (Copyright © 2019. Published by Elsevier Ltd.)