Nybomycin-producing Streptomyces isolated from carpenter ant Camponotus vagus.

Authors :: Zakalyukina YV
Birykov MV
Lukianov DA
Shiriaev DI
Komarova ES
Skvortsov DA
Kostyukevich Y
Tashlitsky VN
Polshakov VI
Nikolaev E
Sergiev PV
Osterman IA
Source :: Biochimie [Biochimie] 2019 May; Vol. 160, pp. 93-99. Date of Electronic Publication: 2019 Feb 21.
Publication Year :: 2019
Abstract: A novel strain of Actinomycetes was isolated from the body of an ant (Camponotus vagus Scopoli) and its genetic and morphological properties were characterized. The 16S rDNA gene sequence analysis of the isolate revealed its high phylogenetic relationship with type strains of Streptomyces violaceochromogenes NBRC 13100 <superscript>T</superscript> . As a result of antimicrobial activity assessment, it was found that the fermentation broth of the isolated strain both inhibited the growth and induced the SOS response in E. coli BW25113 ΔtolC strain cells. Using bioassay-guided fractionation, mass spectrometric and NMR analyses we identified the active compound to be nybomycin, a previously described antibiotic. Here we report for the first time Streptomyces producer of nybomycin in association with carpenter ants and demonstrate cytotoxic activity of nybomycin against human cell lines.<br /> (Copyright © 2019 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.)