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CIK cell-based delivery of recombinant adenovirus KGHV500 carrying the anti-p21Ras scFv gene enhances the anti-tumor effect and safety in lung cancer.
- Source :
-
Journal of cancer research and clinical oncology [J Cancer Res Clin Oncol] 2019 May; Vol. 145 (5), pp. 1123-1132. Date of Electronic Publication: 2019 Feb 22. - Publication Year :
- 2019
-
Abstract
- Purpose: Adenovirus (Ads) is one of the most popular vectors used in gene therapy for the treatment of cancer. However, systemic therapy is limited by circulating antiviral antibodies and poor viral delivery in vivo. In this study, we used cytokine-induced killer (CIK) cells as delivery vehicles of Ads KGHV500 carrying the anti-p21Ras scFv gene to treat Ras gene-related lung cancer and investigate the anti-tumor effect in vitro and in vivo.<br />Methods: The human lung cancer cell line A549 was employed to investigate the anti-tumor activity of recombinant Ads KGHV500 harboring the anti-p21Ras scFv gene using MTT, wound healing, transwell invasion, and apoptosis assays in vitro. Next, CIK cells were used as delivery vehicles to deliver KGHV500 carrying the anti-p21Ras scFv gene to treat A549-transplanted tumors in nude mice, and viral replication, p21Ras scFv expression, and the therapeutic efficacy were assessed.<br />Results: In vitro studies showed that KGHV500 had potent anti-tumor activity. In addition, in vivo, this combination therapy significantly inhibited the growth of lung cancer xenografts compared with mice treated with KGHV500 alone. KGHV500 and anti-p21Ras scFv were observed in tumor tissue, but were nearly undetectable in normal tissues.<br />Conclusions: The co-delivery of anti-p21Ras scFv by CIK cells and KGHV500 could increase the anti-tumor effect and safety, and possess considerable advantages for the treatment of Ras-related cancer.
- Subjects :
- Animals
Apoptosis genetics
Cell Line, Tumor
Cytotoxicity Tests, Immunologic
Disease Models, Animal
Female
Gene Expression
Genetic Therapy
Genetic Vectors genetics
Humans
Immunohistochemistry
Immunotherapy, Adoptive
Lung Neoplasms pathology
Lung Neoplasms therapy
Mice
Transduction, Genetic
Xenograft Model Antitumor Assays
Adenoviridae genetics
Cytokine-Induced Killer Cells immunology
Cytokine-Induced Killer Cells metabolism
Lung Neoplasms genetics
Lung Neoplasms immunology
Proto-Oncogene Proteins p21(ras) immunology
Single-Chain Antibodies genetics
Single-Chain Antibodies immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-1335
- Volume :
- 145
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of cancer research and clinical oncology
- Publication Type :
- Academic Journal
- Accession number :
- 30796510
- Full Text :
- https://doi.org/10.1007/s00432-019-02857-8