Potential biomarkers for antidiastole of tuberculous and malignant pleural effusion by proteome analysis.

Authors :: Shi J
Li P
Zhou L
Qi S
Wang B
Li D
Duan L
Chen WX
Xia J
Zou L
Yang S
Source :: Biomarkers in medicine [Biomark Med] 2019 Feb; Vol. 13 (2), pp. 123-133. Date of Electronic Publication: 2019 Feb 22.
Publication Year :: 2019
Abstract: Aim: To investigate novel potential biomarkers for antidiastole of tuberculous pleural effusion (TPE) from malignant pleural effusion (MPE).<br />Materials & Methods: iTRAQTM-coupled LC-MS/MS were applied to analyze the proteome of TPE and MPE samples. The candidate proteins were verified by enzyme-linked immunosorbent assay.<br />Results: A total of 432 differential proteins were identified. Enzyme-linked immunosorbent assay revealed significantly higher levels of fibronectin (FN) and cathepsin G (CTSG) in MPE than in TPE, but lower levels of leukotriene-A4 hydrolase (LTA4H). The receiver operator characteristic values were 0.285 for FN, 0.64 for LTA4H, 0.337 for CTSG and 0.793 for a combination of these candidate markers.<br />Conclusion: FN, LTA4H and CTSG were identified as potential biomarkers to differentiate TPE from MPE and their combination exhibited higher diagnostic capacity.

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