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AtU2AF65b functions in abscisic acid mediated flowering via regulating the precursor messenger RNA splicing of ABI5 and FLC in Arabidopsis.
- Source :
-
The New phytologist [New Phytol] 2019 Jul; Vol. 223 (1), pp. 277-292. Date of Electronic Publication: 2019 Mar 18. - Publication Year :
- 2019
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Abstract
- In mammalians and yeast, the splicing factor U2AF65/Mud2p functions in precursor messenger RNA (pre-mRNA) processing. Arabidopsis AtU2AF65b encodes a putative U2AF65 but its specific functions in plants are unknown. This paper examines the function of AtU2AF65b as a negative regulator of flowering time in Arabidopsis. We investigated the expression and function of AtU2AF65b in abscisic acid (ABA)-regulated flowering as well as the transcript abundance and pre-mRNA splicing of flowering-related genes in the knock-out mutants of AtU2AF65b. The atu2af65b mutants show early-flowering phenotype under both long-day and short-day conditions. The transcript accumulation of the flowering repressor gene FLOWERING LOCUS C (FLC) is reduced in the shoot apex of atu2af65b, due to both increased intron retention and reduced transcription activation. Reduced transcription of FLC results, at least partially, from the abnormal splicing and reduced transcript abundance of ABSCISIC ACID-INSENSITIVE 5 (ABI5), which encodes an activator of FLC in ABA-regulated flowering signaling. Additionally, the expression of AtU2AF65b is promoted by ABA. Transition to flowering and splicing of FLC and ABI5 in the atu2af65b mutants are compromised during ABA-induced flowering. ABA-responsive AtU2AF65b functions in the pre-mRNA splicing of FLC and ABI5 in shoot apex, whereby AtU2AF65b is involved in ABA-mediated flowering transition in Arabidopsis.<br /> (© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.)
- Subjects :
- Arabidopsis Proteins genetics
Basic-Leucine Zipper Transcription Factors metabolism
Cell Nucleus metabolism
Gene Expression Regulation, Plant
Introns genetics
MADS Domain Proteins metabolism
Mutation genetics
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins metabolism
Seedlings metabolism
Splicing Factor U2AF metabolism
Transcription, Genetic
Up-Regulation genetics
Abscisic Acid metabolism
Arabidopsis genetics
Arabidopsis Proteins metabolism
Basic-Leucine Zipper Transcription Factors genetics
Flowers physiology
MADS Domain Proteins genetics
RNA Splicing genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8137
- Volume :
- 223
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The New phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 30790290
- Full Text :
- https://doi.org/10.1111/nph.15756