Back to Search
Start Over
MicroRNA-1269a promotes the occurrence and progression of osteosarcoma by inhibit-ing TGF-β1 expression.
- Source :
-
European review for medical and pharmacological sciences [Eur Rev Med Pharmacol Sci] 2019 Feb; Vol. 23 (3), pp. 972-981. - Publication Year :
- 2019
-
Abstract
- Objective: MicroRNAs are endogenous, non-coding small RNAs that are capable of regulating biological and pathological processes. Previous studies have shown that microRNA-1269a serves as an oncogene. However, the role of microRNA-1269a in the pathogenesis of osteosarcoma (OS) has not been reported. The aim of this work was to investigate the expression characteristics of microRNA-1269a in OS and to further study its regulatory effects on the malignant progression of OS.<br />Patients and Methods: The expression of microRNA-1269a in 61 pairs of OS tissues and para-cancerous tissues was detected by quantitative Real Time-polymerase Chain Reaction (qRT-PCR). Chi-square test was used to analyze the relationship between microRNA-1269a expression and the characteristics of OS patients, including age, sex, clinical stage and distant metastasis. Subsequently, microRNA-1269a expression in OS cell lines was detected as well. After knockdown of microRNA-1269a by constructing relevant small interference RNA, biological performances of MG63 and U2OS cells were accessed by cell counting kit-8 (CCK-8), colony formation and transwell assay. Meanwhile, the protein expressions of key genes in the EMT/Smad pathway were detected by Western blot. Finally, si-TGF-β1 (transforming growth factor-β1) was transfected into OS cells, and cell migration and invasion were detected by transwell assay.<br />Results: MicroRNA-1269a was highly expressed in OS tissues compared with para-cancerous tissues. High expression of microRNA-1269a was positively correlated with young OS patients and high rate of distant metastasis, whereas was not correlated with age, sex and Enneking stage. Kaplan-Meier survival curves showed that high expression of microRNA-1269a was significantly associated with poor prognosis of OS. The knockdown of microRNA-1269a in MG63 and U2OS cells significantly inhibited cell proliferation, migration and invasion. Meanwhile, microRNA-1269a knockdown in OS cells markedly downregulated the expressions of TGF-β1, p-Smad2, p-Smad3, N-cad, Vimentin and MMP9. Furthermore, TGF-β1 knockdown remarkably decreased migratory and invasive abilities of OS cells.<br />Conclusions: MicroRNA-1269a is highly expressed in OS, which is remarkably correlated with tumor stage, distant metastasis and poor prognosis of OS. In addition, microRNA-1269a promotes the malignant progression of OS by regulating TGF-β1 expression.
- Subjects :
- Cell Line, Tumor
Cell Movement
Cell Proliferation
Disease Progression
Gene Expression Regulation, Neoplastic drug effects
Gene Expression Regulation, Neoplastic physiology
Humans
MicroRNAs biosynthesis
Neoplasm Invasiveness physiopathology
Osteosarcoma diagnosis
Osteosarcoma metabolism
Prognosis
RNA, Small Interfering pharmacology
Transfection
Transforming Growth Factor beta1 genetics
Tumor Stem Cell Assay
MicroRNAs physiology
Osteosarcoma physiopathology
Transforming Growth Factor beta1 biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 2284-0729
- Volume :
- 23
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- European review for medical and pharmacological sciences
- Publication Type :
- Academic Journal
- Accession number :
- 30779063
- Full Text :
- https://doi.org/10.26355/eurrev_201902_16984