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Corticosteroids protect infected cells against mycobacterial killing in vitro.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2019 Mar 26; Vol. 511 (1), pp. 117-121. Date of Electronic Publication: 2019 Feb 14. - Publication Year :
- 2019
-
Abstract
- The effect of corticosteroids on human physiology is complex and their use in tuberculosis patients remains controversial. In a high-throughput screening approach designed to discover virulence inhibitors, several corticosteroids were found to prevent cytolysis of fibroblasts infected with mycobacteria. Further experiments with Mycobacterium tuberculosis showed anti-cytolytic activity in the 10 nM range, but no effect on bacterial growth or survival in the absence of host cells at 20 μM. The results from a panel of corticosteroids with various affinities to the glucocorticoid- and mineralocorticoid receptors indicate that the inhibition of cytolysis most likely is mediated through the glucocorticoid receptor. Using live-imaging of M. tuberculosis-infected human monocyte-derived macrophages, we also show that corticosteroids to some extent control intracellular bacteria. In vitro systems with reduced complexity are to further study and understand the interactions between bacterial infection, immune defense and cell signaling.<br /> (Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Antitubercular Agents pharmacology
Cell Line
Cell Survival drug effects
Cells, Cultured
Fibroblasts cytology
Fibroblasts metabolism
Fibroblasts microbiology
Humans
Macrophages cytology
Macrophages metabolism
Macrophages microbiology
Mycobacterium tuberculosis drug effects
Receptors, Glucocorticoid metabolism
Tuberculosis metabolism
Tuberculosis microbiology
Adrenal Cortex Hormones pharmacology
Fibroblasts drug effects
Macrophages drug effects
Protective Agents pharmacology
Tuberculosis drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 511
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 30773257
- Full Text :
- https://doi.org/10.1016/j.bbrc.2019.02.044