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Corticosteroids protect infected cells against mycobacterial killing in vitro.

Authors :
Tükenmez H
Edström I
Kalsum S
Braian C
Ummanni R
Fick SB
Sundin C
Lerm M
Elofsson M
Larsson C
Source :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2019 Mar 26; Vol. 511 (1), pp. 117-121. Date of Electronic Publication: 2019 Feb 14.
Publication Year :
2019

Abstract

The effect of corticosteroids on human physiology is complex and their use in tuberculosis patients remains controversial. In a high-throughput screening approach designed to discover virulence inhibitors, several corticosteroids were found to prevent cytolysis of fibroblasts infected with mycobacteria. Further experiments with Mycobacterium tuberculosis showed anti-cytolytic activity in the 10 nM range, but no effect on bacterial growth or survival in the absence of host cells at 20 μM. The results from a panel of corticosteroids with various affinities to the glucocorticoid- and mineralocorticoid receptors indicate that the inhibition of cytolysis most likely is mediated through the glucocorticoid receptor. Using live-imaging of M. tuberculosis-infected human monocyte-derived macrophages, we also show that corticosteroids to some extent control intracellular bacteria. In vitro systems with reduced complexity are to further study and understand the interactions between bacterial infection, immune defense and cell signaling.<br /> (Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1090-2104
Volume :
511
Issue :
1
Database :
MEDLINE
Journal :
Biochemical and biophysical research communications
Publication Type :
Academic Journal
Accession number :
30773257
Full Text :
https://doi.org/10.1016/j.bbrc.2019.02.044