Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers.

Authors :: Roberts RF
Bengoa-Vergniory N
Alegre-Abarrategui J
Source :: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1948, pp. 69-76.
Publication Year :: 2019
Abstract: Alpha-synuclein oligomers are thought to be toxic mediators of Parkinson's disease and other alpha-synucleinopathies, but their histological detection in situ in diseased brain has been a challenge in the field for some time. Here we describe a method, the alpha-synuclein proximity ligation assay (AS-PLA), to detect alpha-synuclein oligomers in paraffin-embedded brain sections. Using AS-PLA previously unobserved alpha-synuclein oligomeric pathology is revealed.

Subjects :: Humans
Parkinson Disease metabolism
Parkinson Disease pathology
Protein Aggregates
Protein Aggregation, Pathological metabolism
Brain metabolism
Protein Multimerization
alpha-Synuclein chemistry
alpha-Synuclein metabolism

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