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Effect of essential oil from Ageratum fastigiatum on beta-integrin (CD18) expression on human lymphocytes stimulated with phorbol myristate acetate in vitro.
- Source :
-
Natural product research [Nat Prod Res] 2020 Dec; Vol. 34 (23), pp. 3409-3413. Date of Electronic Publication: 2019 Feb 13. - Publication Year :
- 2020
-
Abstract
- Agareratum fastigiatum is a Brazilian medicinal plant used as anti-inflammaroty and for wound healing by the folk medicine. In vitro and in vivo studies involving A. fastigiatum essential oil (EOAF) showed indications of anti-inflammatory activity, however, its effect on membrane integrins involved on cell migration is still unclear. Hence, it was evaluated in the present study the effect of EOAF on CD18 frequency on human lymphocytes. By using gas chromatography/mass spectrometry it was identified 9 compounds on EOAF: α-pinene; β-pinene; β-myrcene; d-limonene; β-ocimene; sesquiterpenes; α-copaene; 4,8-β-epóxi-caryophyllene; germacrene and bicyclogermacrene. On in vitro tests, 6.25 × 10 <superscript>-3</superscript> and 12.5 × 10 <superscript>-3</superscript> µL/mL EOAF reduced CD18 frequency on phorbol-12-myristate-13-acetate (PMA)-stimulated lymphocytes. Such cells were obtained from peripheral blood of healthy volunteers, and were treated or not with EOAF. They were stained with fluorescent anti-CD18 monoclonal antibodies, after 24 hours incubation. Our data corroborates previous findings, indicating a possible anti-inflammatory activity of EOAF.
- Subjects :
- Acyclic Monoterpenes analysis
Alkenes analysis
Bicyclic Monoterpenes analysis
Gas Chromatography-Mass Spectrometry
Humans
Limonene analysis
Monoterpenes analysis
Oils, Volatile analysis
Plants, Medicinal chemistry
Sesquiterpenes analysis
Tetradecanoylphorbol Acetate pharmacology
Ageratum chemistry
CD18 Antigens metabolism
Lymphocytes drug effects
Lymphocytes metabolism
Oils, Volatile chemistry
Oils, Volatile pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1478-6427
- Volume :
- 34
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- Natural product research
- Publication Type :
- Academic Journal
- Accession number :
- 30758988
- Full Text :
- https://doi.org/10.1080/14786419.2019.1569653