Lysobacter psychrotolerans sp. nov., isolated from soil in the Tianshan Mountains, Xinjiang, China.

A novel aerobic bacterial strain, designated ZS60 ^T , with long, rod-shaped, Gram-staining-negative, aerobic cells was isolated from the soil in the Tianshan Mountains, Xinjiang, China. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain ZS60 ^T was affiliated with the genus Lysobacter, and was most closely related to Lysobacter daejeonensis GH1-9 ^T (96.9 %), Lysobacter caeni BUT-8 ^T (96.8 %) and Lysobacter ruishenii CTN-1 ^T (96.7 %). The average nucleotide identity values between strain ZS60 ^T , L. daejeonensis GH1-9 ^T and L. ruishenii CTN-1 ^T were 78.14 and 78.39 %, respectively. The DNA-DNA relatedness between strain ZS60 ^T , L. daejeonensis GH1-9 ^T and L. caeni BUT-8 ^T were 44.8 and 39.1 %, respectively. The genomic DNA G+C content of the strain ZS60 ^T was 67.7 mol% (draft genome sequence), and Q-8 was the predominant ubiquinone. The major cellular fatty acids of strain ZS60 ^T were iso-C15 : 0 (23.4 %), iso-C17 : 0 (17.2 %) and iso-C17 : 1 ω9c (12.6 %). On the basis of genotypic, phenotypic and biochemical data, strain ZS60 ^T is considered to represent a novel species of the genus Lysobacter, for which the name Lysobacterpsychrotolerans sp. nov. is proposed. The type strain is ZS60 ^T (=CGMCC 1.15509 ^T =NBRC 112614 ^T ).