Method to quantify cytokines and chemokines in mouse brain tissue using Bio-Plex multiplex immunoassays.

Authors :: Manglani M
Rua R
Hendricksen A
Braunschweig D
Gao Q
Tan W
Houser B
McGavern DB
Oh K
Source :: Methods (San Diego, Calif.) [Methods] 2019 Apr 01; Vol. 158, pp. 22-26. Date of Electronic Publication: 2019 Feb 10.
Publication Year :: 2019
Abstract: This protocol describes how to prepare mouse brain tissue for quantification of multiple inflammatory mediators using a multiplex bead-based immunoassay. It is important to have methods that allow quantification of multiple analytes from small amounts of tissue. Bio-Plex is a Luminex xMAP-based multiplex bead-based immunoassay technology that permits simultaneous analysis of up to 100 analytes from a single tissue sample. This assay has been used extensively to investigate analytes in plasma and serum samples as well as cultured and primary cells. Here, we describe a method for simultaneous analysis of 33 different inflammatory cytokines and chemokines from mouse brain tissue using the Bio-Plex Pro Mouse Chemokine Panel 33-Plex.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)

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