Back to Search
Start Over
Pb exposure reduces the expression of SNX6 and Homer1 in offspring rats and PC12 cells.
- Source :
-
Toxicology [Toxicology] 2019 Mar 15; Vol. 416, pp. 23-29. Date of Electronic Publication: 2019 Feb 06. - Publication Year :
- 2019
-
Abstract
- Lead (Pb) is a widespread environmental heavy metal toxicant and chronic Pb exposure can have irreversible effects on memory and cognitive function, which is closely related to dendritic spines. Studies have shown that SNX6 and Homer1 can regulate the growth of dendritic spines. We aimed to investigate the effect of Pb exposure on the dendritic spines in hippocampus, the expression of SNX6 and Homer1 in rats and PC12 cells. The animals were randomly divided to three groups: control group, low lead group and high lead group. PC12 cells were divided into 3 groups: 0 μM, 1 μM and 100 μM Pb acetate. The results showed that the Pb levels in blood and hippocampus of all exposure groups were significantly higher than that of the control group. The morphology of dendritic spines in hippocampus after Pb treatment was changed and the density of dendritic spines was reduced. The expression of SNX6 and Homer1 was decreased in Pb exposed groups compared with the control group. Furthermore, up-regulation of SNX6 expression could reverse the down-regulation of Pb exposure on Homer1. These results indicate that Pb exposure can reduce the expression of SNX6 and lead to a decrease in Homer1 expression, which affects the changes in dendritic spines causing learning and memory impairment.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Dendritic Spines drug effects
Dendritic Spines metabolism
Dendritic Spines pathology
Down-Regulation
Hippocampus metabolism
Hippocampus pathology
Homer Scaffolding Proteins genetics
Lead Poisoning, Nervous System genetics
Lead Poisoning, Nervous System metabolism
Lead Poisoning, Nervous System pathology
Male
PC12 Cells
Pyramidal Cells drug effects
Pyramidal Cells metabolism
Pyramidal Cells pathology
Rats
Rats, Sprague-Dawley
Sorting Nexins genetics
Hippocampus drug effects
Homer Scaffolding Proteins metabolism
Lead Poisoning, Nervous System etiology
Organometallic Compounds toxicity
Sorting Nexins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3185
- Volume :
- 416
- Database :
- MEDLINE
- Journal :
- Toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 30738087
- Full Text :
- https://doi.org/10.1016/j.tox.2019.02.002