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Depletion of cytotoxic (CD8+) T cells impairs implant fixation in rat cancellous bone.
- Source :
-
Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2019 Apr; Vol. 37 (4), pp. 805-811. Date of Electronic Publication: 2019 Feb 28. - Publication Year :
- 2019
-
Abstract
- As cytotoxic (CD8 <superscript>+</superscript> ) T cells seem to impair shaft fracture healing, we hypothesized that depletion of CD8 <superscript>+</superscript> cells would instead improve healing of cancellous bone. Additionally, we also tested if CD8-depletion would influence the healing of ruptured Achilles tendons. Rats received a single injection of either anti-CD8 antibodies or saline and put through surgery 24 h later. Three different surgical interventions were performed as follows: (1) a drill hole in the proximal tibia with microCT (BV/TV) to assess bone formation; (2) a screw in the proximal tibia with mechanical evaluation (pull-out force) to assess fracture healing; (3) Achilles tendon transection with mechanical evaluation (force-at-failure) to assess tendon healing. Furthermore, CD8-depletion was confirmed with flow cytometry on peripheral blood. Flow cytometric analysis confirmed depletion of CD8 <superscript>+</superscript> cells (p < 0.001). Contrary to our hypothesis, depletion of CD8 <superscript>+</superscript> cells reduced the implant pull-out force by 19% (p < 0.05) and stiffness by 34% (p < 0.01), although the bone formation in the drill holes was the same as in the controls. Tendon healing was unaffected by CD8-depletion. Our results suggest that CD8 <superscript>+</superscript> cells have an important part in cancellous bone healing. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.<br /> (© 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.)
Details
- Language :
- English
- ISSN :
- 1554-527X
- Volume :
- 37
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of orthopaedic research : official publication of the Orthopaedic Research Society
- Publication Type :
- Academic Journal
- Accession number :
- 30737834
- Full Text :
- https://doi.org/10.1002/jor.24246