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High efficient differentiation of human adipose-derived stem cells into retinal pigment epithelium-like cells in medium containing small molecules inducers with a simple method.
- Source :
-
Tissue & cell [Tissue Cell] 2019 Feb; Vol. 56, pp. 52-59. Date of Electronic Publication: 2018 Dec 12. - Publication Year :
- 2019
-
Abstract
- Background: The induction of retinal pigmented epithelium cells (RPE) is one of the most important objectives in research focused on treating retinal degenerative diseases. The present study aims to differentiate human adipose stem cells (hADSCs) into RPE cells for replacement therapies in cases of retinal degenerative diseases.<br />Methods: Lipoaspirate-derived human adipose stem cells (LA-hADSCs) were obtained from abdominal samples and examined by immunocytochemistry for the expression of mesenchymal adipose stem cell markers. RPE cells were also obtained from human samples and cultured to be used as control after being examined for the expression of their designated markers. hADSCs differentiated into RPE cells after 80 days using chemical inducers in one steps. The differentiated cells were then compared to control cells in marker expression. The differentiated cells were also examined under a scanning electron microscope for the presence of apical microvilli and cell connection.<br />Results: Cultured hADSCs at the fourth passage was shown to express the surface markers CD90 (98 ± 2%), CD11b (96 ± 3%), and CD105 (95 ± 4%). The RPE cells obtained from human samples expressed the marker RPE65 quite well. 80 days after differentiation, the previously hADSCs expressed both RPE65 (100%) and CRALBP (96 ± 1%) and were thus significantly similar to the RPE cells obtained from human samples. Morphologically, differentiated cells appeared to have epithelial and cytoplasmic pigment granules. Observations using a scanning electron microscope recorded clear connections among the differentiated RPE cells and revealed apical microvilli.<br />Conclusion: Human adipose stem cells can differentiate into retinal pigmented epithelium cells, which can be used in cell replacement therapy for degenerative diseases including age-related macular degeneration (AMD) as well as retinitis pigmentosa (RP).<br /> (Published by Elsevier Ltd.)
- Subjects :
- Cell Shape
Cells, Cultured
Culture Media chemistry
Humans
Mesenchymal Stem Cells drug effects
Neurons drug effects
Neurons transplantation
Retina drug effects
Retina pathology
Retina transplantation
Retinal Degeneration pathology
Retinal Pigment Epithelium cytology
Small Molecule Libraries administration & dosage
Cell Differentiation drug effects
Mesenchymal Stem Cell Transplantation
Mesenchymal Stem Cells cytology
Retinal Degeneration therapy
Retinal Pigment Epithelium transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 1532-3072
- Volume :
- 56
- Database :
- MEDLINE
- Journal :
- Tissue & cell
- Publication Type :
- Academic Journal
- Accession number :
- 30736904
- Full Text :
- https://doi.org/10.1016/j.tice.2018.12.003