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Allogeneic major histocompatibility complex antigens are necessary and sufficient for partial tolerance induced by transfusion of pathogen reduced platelets in mice.
- Source :
-
Vox sanguinis [Vox Sang] 2019 Apr; Vol. 114 (3), pp. 207-215. Date of Electronic Publication: 2019 Feb 07. - Publication Year :
- 2019
-
Abstract
- Background and Objectives: Alloimmunization is common following transfusion with platelet-rich plasma (PRP) and can cause complications such as platelet refractoriness or transplant rejection. It has previously been shown that pathogen reduction of PRP with riboflavin and UV light (UV+R) can protect against alloimmunization in mice and induce partial tolerance to subsequent transfusions.<br />Materials and Methods: Using B6 H2 <superscript>d</superscript> congenic mice, this study evaluated the relative contributions of major histocompatibility complex (MHC) antigens and minor antigens to both the alloresponse to PRP transfusion and the partial tolerance induced by UV+R treatment.<br />Results: Both total and MHC-specific alloantibody responses were highest when both MHC and minor antigens were mismatched, with lower alloantibody responses observed with MHC mismatch alone, demonstrating that allogeneic minor antigens can enhance the response to allogeneic MHC. There was a weak, but significant alloantibody response to minor antigens only. UV+R treatment protected against both major and minor antigen alloimmunization. Both allogeneic MHC and minor antigens primed an enhanced cytokine response ex vivo, though this was weaker with minor antigens, and both responses were blocked with UV+R treatment.<br />Conclusion: Allogeneic MHC is both necessary and sufficient to induce the partial tolerance associated with UV+R treatment.<br /> (© 2019 International Society of Blood Transfusion.)
- Subjects :
- Animals
Blood Platelets drug effects
Blood Platelets radiation effects
Isoantibodies immunology
Mice
Mice, Inbred BALB C
Platelet Transfusion adverse effects
Riboflavin pharmacology
Ultraviolet Rays
Blood Platelets immunology
Immune Tolerance
Major Histocompatibility Complex immunology
Platelet Transfusion methods
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0410
- Volume :
- 114
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Vox sanguinis
- Publication Type :
- Academic Journal
- Accession number :
- 30734299
- Full Text :
- https://doi.org/10.1111/vox.12756