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Dansyl-appended Cu II -complex-based nitroxyl (HNO) sensing with living cell imaging application and DFT studies.
- Source :
-
Dalton transactions (Cambridge, England : 2003) [Dalton Trans] 2019 Feb 19; Vol. 48 (8), pp. 2760-2771. - Publication Year :
- 2019
-
Abstract
- We introduce herein, a novel copper complex-based fluorescent probe [CuII(DQ468)Cl]+ that exhibits a significant fluorescence turn-on response towards nitroxyl (HNO) with high selectivity over other biological reactive oxygen, nitrogen and sulfur species, including nitric oxide (NO). A smart strategy, involving HNO-induced reduction of paramagnetic [CuII(DQ468)Cl]+ to diamagnetic [CuI(DQ468)]+ with concomitant fluorescence enhancement via a PET mechanism is focused here. This reduction-based strategy was also supported by X-band EPR response and mass spectroscopy. The metal free probe (DQ468) showed high affinity towards Cu2+ to form [CuII(DQ468)Cl]+ with a 0.091 μM detection limit, which subsequently enabled the detection of HNO in an organo-aqueous medium at biological pH (7.4) in the green wavelength region (λem = 543 nm) with a LOD of 0.41 μM. The ground-state geometries of DQ468, [CuII(DQ468)Cl]+ and [CuI(DQ468)]+ were optimized by DFT calculations, which revealed that the central metal ion in [CuII(DQ468)Cl]+ is in a distorted tetrahedral geometry with the C1 point group. Additionally, the negligible cytotoxicity and good biocompatibility make the developed probe useful for the in vitro detection of HNO.
Details
- Language :
- English
- ISSN :
- 1477-9234
- Volume :
- 48
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Dalton transactions (Cambridge, England : 2003)
- Publication Type :
- Academic Journal
- Accession number :
- 30724923
- Full Text :
- https://doi.org/10.1039/c8dt04564j