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Gardnerella vaginalis and Prevotella bivia Trigger Distinct and Overlapping Phenotypes in a Mouse Model of Bacterial Vaginosis.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2019 Aug 30; Vol. 220 (7), pp. 1099-1108. - Publication Year :
- 2019
-
Abstract
- Background: Bacterial vaginosis (BV) is a common imbalance of the vaginal microbiota characterized by overgrowth of diverse Actinobacteria, Firmicutes, and Gram-negative anaerobes. Women with BV are at increased risk of secondary reproductive tract infections and adverse pregnancy outcomes. However, which specific bacteria cause clinical features of BV is unclear.<br />Methods: We previously demonstrated that Gardnerella vaginalis could elicit many BV features in mice. In this study, we established a BV model in which we coinfected mice with G. vaginalis and another species commonly found in women with BV: Prevotella bivia.<br />Results: This coinfection model recapitulates several aspects of human BV, including vaginal sialidase activity (a diagnostic BV feature independently associated with adverse outcomes), epithelial exfoliation, and ascending infection. It is notable that G. vaginalis facilitated uterine infection by P. bivia.<br />Conclusions: Taken together, our model provides a framework for advancing our understanding of the role of individual or combinations of BV-associated bacteria in BV pathogenesis.<br /> (© The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.)
- Subjects :
- Animals
Disease Models, Animal
Female
Humans
Mice
Mice, Inbred C57BL
Microbiota
Neuraminidase analysis
RNA, Bacterial genetics
RNA, Ribosomal, 16S genetics
Reverse Transcriptase Polymerase Chain Reaction
Vagina microbiology
Coinfection microbiology
Gardnerella vaginalis genetics
Phenotype
Prevotella genetics
Vaginosis, Bacterial microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1537-6613
- Volume :
- 220
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 30715405
- Full Text :
- https://doi.org/10.1093/infdis/jiy704