Apoptosis Induced by Ultraviolet A Exposure in the Presence of Enoxacin in HL-60 Cells.

Background: The ultraviolet A (UVA) spectrum mainly includes the region associated with the phototoxicity of fluoroquinolone antimicrobial agents. This study investigated apoptosis induced with UVA light and enoxacin in HL-60 cells.
Materials and Methods: HL-60 cells were irradiated by UVA (1.1 mW/cm ² ) for 20 min in the presence or absence of enoxacin. The induction of apoptosis was investigated by analysing cell morphology, flow cytometry of annexin V-positive cells, DNA ladder formation, and caspase-3 activation.
Results: Significant induction of apoptosis, DNA fragmentation, and caspase-3 activation were observed in cells treated with both UVA and enoxacin. UVA-induced apoptosis was significantly suppressed when NaN3, a singlet oxygen scavenger, was present.
Conclusion: Apoptosis was induced by the combination of UVA and enoxacin in HL-60 cells, and singlet oxygen appears to play an important role in photodynamically-induced apoptosis.
(Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)