A two-gene methylation signature for the diagnosis of bladder cancer in urine.

Authors :: Bosschieter J
Nieuwenhuijzen JA
Hentschel A
van Splunter AP
Segerink LI
Vis AN
Wilting SM
Lissenberg-Witte BI
A van Moorselaar RJ
Steenbergen RD
Source :: Epigenomics [Epigenomics] 2019 Feb; Vol. 11 (3), pp. 337-347. Date of Electronic Publication: 2019 Feb 01.
Publication Year :: 2019
Abstract: Aim: To analyze the potential of 14 cancer-associated genes, including six miRNAs, for bladder cancer (BC) diagnosis in urine.<br />Patients & Methods: DNA methylation levels of 14 genes were analyzed in urine of 72 BC patients and 75 healthy controls using quantitative methylation-specific PCR. Multivariate logistic regression analysis was used to determine an optimal marker panel.<br />Results: Ten genes were significantly hypermethylated in BC patients. The GHSR/MAL combination showed the best diagnostic performance, reaching a sensitivity of 92% (95% CI: 86-99) and a specificity of 85% (95% CI: 76-94).<br />Conclusion: We identified a novel two-gene panel with a high diagnostic accuracy for BC that can be applied in a noninvasive, urine-based test.