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CSRP3 mediates polyphenols-induced cardioprotection in hypertension.
- Source :
-
The Journal of nutritional biochemistry [J Nutr Biochem] 2019 Apr; Vol. 66, pp. 29-42. Date of Electronic Publication: 2019 Jan 08. - Publication Year :
- 2019
-
Abstract
- Berries contain bioactive polyphenols, whose capacity to prevent cardiovascular diseases has been established recently in animal models as well in human clinical trials. However, cellular processes and molecular targets of berries polyphenols remain to be identified. The capacity of a polyphenol-enriched diet (i.e., blueberries, blackberries, raspberries, strawberry tree fruits and Portuguese crowberries berries mixture) to promote animal survival and protect cardiovascular function from salt-induced hypertension was evaluated in a chronic salt-sensitive Dahl rat model. The daily consumption of berries improved survival of Dahl/salt-sensitive rats submitted to high-salt diet and normalized their body weight, renal function and blood pressure. In addition, a prophylactic effect was observed at the level of cardiac hypertrophy and dysfunction, tissue cohesion and cardiomyocyte hypertrophy. Berries also protected the aorta from fibrosis and modulated the expression of aquaporin-1, a channel involved in endothelial water and nitric oxide permeability. Left ventricle proteomics analysis led to the identification of berries and salt metabolites targets, including cystein and glycin-rich protein 3 (CSRP3), a protein involved in myocyte cytoarchitecture. In neonatal rat ventricular cardiomyocytes, CSRP3 was validated as a target of a berries-derived polyphenol metabolite, 4-methylcatechol sulfate, at micromolar concentrations, mimicking physiological conditions of human plasma circulation. Accordingly, siRNA silencing of CSRP3 and 4-methylcatechol sulfate pretreatment reversed cardiomyocyte hypertrophy and CSRP3 overexpression induced by phenylephrine. Our systemic study clearly supports the modulation of CSRP3 by a polyphenol-rich berries diet as an efficient cardioprotective strategy in hypertension-induced heart failure.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cardiomegaly diet therapy
Cardiomegaly prevention & control
Cells, Cultured
Disease Models, Animal
Heart drug effects
Hypertension mortality
LIM Domain Proteins genetics
Male
Muscle Proteins genetics
Myocytes, Cardiac drug effects
Myocytes, Cardiac metabolism
Myocytes, Cardiac pathology
Rats, Inbred Dahl
Cardiotonic Agents pharmacology
Fruit
Hypertension diet therapy
LIM Domain Proteins metabolism
Muscle Proteins metabolism
Polyphenols pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4847
- Volume :
- 66
- Database :
- MEDLINE
- Journal :
- The Journal of nutritional biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 30703746
- Full Text :
- https://doi.org/10.1016/j.jnutbio.2019.01.001