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In-vitro viral suppressive capacity correlates with immune checkpoint marker expression on peripheral CD8+ T cells in treated HIV-positive patients.
- Source :
-
AIDS (London, England) [AIDS] 2019 Mar 01; Vol. 33 (3), pp. 387-398. - Publication Year :
- 2019
-
Abstract
- Objective: To determine whether viral suppressive capacity (VSC) of CD8+ T cells can be boosted by stimulation with HIV-1 peptides and whether the ability to control HIV-1 replication correlates with immunological (cytokine production and CD8+ T-cell phenotype) and viral reservoir measures (total HIV-1 DNA and cell-associated RNA) in well treated HIV-infected chronic progressors.<br />Design: We compared VSC of peripheral CD8+ T cells to cytokine production profile in response to peptide stimulation, detailed phenotype (17-color flow-cytometry), reservoir size (total HIV-1 DNA), basal viral transcription (unspliced cell-associated RNA) and inducible viral transcription (tat/rev induced limiting dilution assay) in 36 HIV+ patients on cART and six healthy donors.<br />Results: We found that the VSC of CD8+ T cells can be increased by prior stimulation with a pool of consensus HIV-1 gag peptides in a significant proportion of progressor patients. We also found that VSC after peptide stimulation was correlated with higher expression of immune checkpoint markers on subsets of terminally differentiated effector memory (TEMRA) CD8 T cells as well as with production of IFN-γ, TNF-α and IL-10. We did not find a correlation between VSC and viral reservoir measures.<br />Conclusion: These results add to a small body of evidence that the capacity of CD8+ T cells to suppress viral replication is increased after stimulation with HIV-1 peptides. Interestingly, this VSC was correlated with expression of immune checkpoint markers, which are generally considered to be markers of exhaustion. Our findings may guide further investigations into immune phenotypes correlated with viral suppression.
- Subjects :
- Adult
Anti-HIV Agents pharmacology
Cytokines analysis
DNA, Viral analysis
Female
HIV-1 growth & development
HIV-1 immunology
Humans
Male
Middle Aged
RNA, Viral analysis
Transcription, Genetic
Treatment Outcome
Viral Proteins administration & dosage
Virus Replication drug effects
AIDS Vaccines administration & dosage
Anti-HIV Agents therapeutic use
Biomarkers analysis
CD8-Positive T-Lymphocytes immunology
HIV Infections drug therapy
HIV Infections immunology
HIV-1 drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1473-5571
- Volume :
- 33
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- AIDS (London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 30702513
- Full Text :
- https://doi.org/10.1097/QAD.0000000000002068