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Targeted Metagenomics of Microbial Diversity in Free-Living Amoebae and Water Samples.

Authors :
Delafont V
Perrin Y
Bouchon D
Moulin L
Héchard Y
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1921, pp. 421-428.
Publication Year :
2019

Abstract

The presence of Legionella spp. in natural and man-made water systems is a great public health concern and heavily depends on the presence of free-living amoebae. Taking advantage of the development and affordability of next-generation sequencing technology, we present here a method to characterize the whole bacterial community directly from water samples, as well as from isolated free-living amoebae.

Details

Language :
English
ISSN :
1940-6029
Volume :
1921
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
30694507
Full Text :
https://doi.org/10.1007/978-1-4939-9048-1_26