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Maturing Human CD127+ CCR7+ PDL1+ Dendritic Cells Express AIRE in the Absence of Tissue Restricted Antigens.
- Source :
-
Frontiers in immunology [Front Immunol] 2019 Jan 14; Vol. 9, pp. 2902. Date of Electronic Publication: 2019 Jan 14 (Print Publication: 2018). - Publication Year :
- 2019
-
Abstract
- Expression of the Autoimmune regulator (AIRE) outside of the thymus has long been suggested in both humans and mice, but the cellular source in humans has remained undefined. Here we identify AIRE expression in human tonsils and extensively analyzed these "extra-thymic AIRE expressing cells" (eTACs) using combinations of flow cytometry, CyTOF and single cell RNA-sequencing. We identified AIRE+ cells as dendritic cells (DCs) with a mature and migratory phenotype including high levels of antigen presenting molecules and costimulatory molecules, and specific expression of CD127, CCR7, and PDL1. These cells also possessed the ability to stimulate and re-stimulate T cells and displayed reduced responses to toll-like receptor (TLR) agonists compared to conventional DCs. While expression of AIRE was enriched within CCR7+CD127+ DCs, single-cell RNA sequencing revealed expression of AIRE to be transient, rather than stable, and associated with the differentiation to a mature phenotype. The role of AIRE in central tolerance induction within the thymus is well-established, however our study shows that AIRE expression within the periphery is not associated with an enriched expression of tissue-restricted antigens (TRAs). This unexpected finding, suggestive of wider functions of AIRE, may provide an explanation for the non-autoimmune symptoms of APECED patients who lack functional AIRE.
- Subjects :
- B7-H1 Antigen metabolism
Cell Communication immunology
Cells, Cultured
Child, Preschool
Dendritic Cells metabolism
Humans
Infant
Infant, Newborn
Interleukin-7 Receptor alpha Subunit metabolism
Lymphocyte Activation
Palatine Tonsil immunology
Palatine Tonsil metabolism
Primary Cell Culture
Receptors, CCR7 metabolism
T-Lymphocytes immunology
Transcription Factors immunology
AIRE Protein
Dendritic Cells immunology
Palatine Tonsil cytology
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 9
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 30692988
- Full Text :
- https://doi.org/10.3389/fimmu.2018.02902