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Regulated expression of Gemin5, Xrn1, Cpeb and Stau1 in the uterus and ovaries after superovulation and the effect of exogenous estradiol and leptin in rodents.
- Source :
-
Molecular biology reports [Mol Biol Rep] 2019 Apr; Vol. 46 (2), pp. 2533-2540. Date of Electronic Publication: 2019 Jan 28. - Publication Year :
- 2019
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Abstract
- The aim of this study was to evaluate whether Gemin 5, Cpeb, Xrn1, and Stau1 expression in rodent ovaries and uterine tissues is dependent on gonadotropins, steroid hormones, and leptin in the superovulation and ovariectomized mouse models of menopause. Treatment of pregnant mare serum gonadotropin-primed rats with human chorionic gonadotropin (hCG) significantly induced Stau1 and Gemin 5 messenger RNA expression in rat ovaries. Gemin 5 expression in ovaries was sustained at relatively high levels at 12 h and 24 h post hCG treatment compared to Stau1, suggesting its role in follicle development, ovulation, and luteogenesis in rat ovaries. Induced expression of Stau1 and Gemin 5 in the uterine tissue post hCG treatment at 12 h and 24 h-the duration between ovulation and post-ovulation-suggests their regulation by hCG and/or ovarian steroids, which are required for pregnancy establishment and maintenance. Cpeb expression was significantly higher (pā<ā0.05) in the uterine tissues after combined treatment of estradiol and leptin at 4 h. Further, the significant upregulation of uterine Gemin 5 and Xrn1 by the synergistic activities of leptin and estradiol at 40 h in ovariectomized mice establishes them as targets of cross-talk. Although these are preliminary data, the combination of Gemin 5, Cpeb, Xrn1, and Stau1 transcript alterations in rodent ovaries and uterine tissue displayed in two different experimental models underscore their importance as therapeutic targets for anovulation or in overcoming endometrial homeostasis disturbances during pregnancy due to obesity.
- Subjects :
- Animals
Chorionic Gonadotropin
Cytoskeletal Proteins metabolism
Estradiol metabolism
Exoribonucleases genetics
Exoribonucleases metabolism
Female
Gene Expression Regulation genetics
Leptin metabolism
Microtubule-Associated Proteins metabolism
Ovariectomy
Ovary metabolism
Ovary pathology
Progesterone blood
RNA-Binding Proteins genetics
Rats
Rats, Wistar
SMN Complex Proteins genetics
Superovulation
Uterus drug effects
Uterus metabolism
Uterus pathology
mRNA Cleavage and Polyadenylation Factors genetics
mRNA Cleavage and Polyadenylation Factors metabolism
RNA-Binding Proteins metabolism
SMN Complex Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-4978
- Volume :
- 46
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular biology reports
- Publication Type :
- Academic Journal
- Accession number :
- 30689188
- Full Text :
- https://doi.org/10.1007/s11033-019-04606-z